rabbit anti zif268  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Parity and APOEε4 genotype contribute distinct changes to functional connectivity across the middle-aged brain

doi: 10.1101/2025.03.01.640999

Figure Lengend Snippet: Average density of zif268-IR cells ± standard error of the mean in the (A) medial dorsal striatum, (B) lateral dorsal striatum, (C) nucleus accumbens shell, and (D) nucleus accumbens core. Zif268 cell density was the lowest in primiparous hAPOEε4 rats compared to all other groups in all subregions of the dorsal striatum and nucleus accumbens. Average density of zif268-IR cells ± standard error of the mean in the (E) prelimbic cortex, (F) infralimbic cortex, and (G) anterior cingulate cortex. Across all subregions of the frontal cortex, zif268 cell density was lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats and in hAPOEε4 rats compared to wildtype rats. Zif268 cell density was greater in the ACC compared to PrL and IL. Average density of zif268-IR cells ± standard error of the mean in the (H) granular retrosplenial cortex C and (I) granular retrosplenial cortex AB. Zif268 cell density was the lower in primiparous hAPOEε4 rats compared to primiparous wildtype rats in the granular retrosplenial cortex. Average density of zif268-IR cells ± standard error of the mean in the (J) dorsal CA1, (K) dorsal DG, and (L) ventral DG. Zif268 cell density in the dorsal CA1 was lower in primiparous rats compared to nulliparous rats. Of all hippocampal subregions, zif2668 cell density was lowest in the dentate gyrus, with lower density in the dDG compared to vDG. * indicates p <0.05. CA – cornu ammonis, DG – dentate gyrus, zif268 – zinc finger-containing transcription factor 268, IR – immunoreactive, hAPOEε4 – humanized APOEε4

Article Snippet: Tissue was incubated in primary antibody solution containing 1:500 goat anti-DCX (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 1:1000 rabbit anti-zif268 (Egr-1 SC-189; Santa Cruz Biotechnology, Santa Cruz, CA, USA) in a dilution solution containing 1% normal donkey serum and 0.3% Triton-X in 0.1M PBS for 24 hours at 4°C.

Techniques:

Journal: bioRxiv

Article Title: Parity and APOEε4 genotype contribute distinct changes to functional connectivity across the middle-aged brain

doi: 10.1101/2025.03.01.640999

Figure Lengend Snippet: (A-D) Heat maps generated based on correlation coefficients between density of zif268-IR cells in the different regions of interest and percentage of DCX/zif268 co-labelled cells in the dorsal and ventral dentate gyrus across groups. All negative (blue) and positive (red) correlations are included, and boxes with text indicate those with a coefficient | r | > 0.5. (E-H) Circular network maps and (I-L) geometric network maps were generated based on correlation coefficients between density of zif268-IR cells in the different regions of interest and percentage of DCX/zif268 co-labelled cells in the dorsal and ventral dentate gyrus across groups. Negative (blue) and positive (red) correlations with coefficient | r | > 0.5 were included. hAPOEε4 – humanized APOEε4

Article Snippet: Tissue was incubated in primary antibody solution containing 1:500 goat anti-DCX (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 1:1000 rabbit anti-zif268 (Egr-1 SC-189; Santa Cruz Biotechnology, Santa Cruz, CA, USA) in a dilution solution containing 1% normal donkey serum and 0.3% Triton-X in 0.1M PBS for 24 hours at 4°C.

Techniques: Generated

Journal: Neural Plasticity

Article Title: Plasticity-Related PKM ζ Signaling in the Insular Cortex Is Involved in the Modulation of Neuropathic Pain after Nerve Injury

doi: 10.1155/2015/601767

Figure Lengend Snippet: Fluorescence images of zif268 expression in the IC of nerve-injured and sham groups. (a) The sham group showed little expression of zif268-positive cells, unlike the nerve-injured group. (b) NeuN, a neuronal marker (red), was expressed in the sham group. (c) Colocalization of zif268 (green) and NeuN (red) is observed in the sham group. (d) In the nerve-injured group, the distribution of zif268 expression (green) was denser than in the sham group. (e) As in (b), NeuN was expressed in the nerve-injured group. (f) As in (c), colocalization of zif268 (green) and NeuN (red) is observed in the nerve-injured group. Scale bar, 50 μ m.

Article Snippet: After washing the section slides 5 times, the sections were incubated with PBS containing 1% normal horse serum (Vector Laboratories) for 30 minutes and then incubated overnight at 4°C in 0.3% Triton X-100, 2% normal horse serum (Vector Laboratories), and rabbit monoclonal anti-zif268 antibody (1 : 4,000, Santa Cruz Biotechnology).

Techniques: Fluorescence, Expressing, Marker

Journal: Neural Plasticity

Article Title: Plasticity-Related PKM ζ Signaling in the Insular Cortex Is Involved in the Modulation of Neuropathic Pain after Nerve Injury

doi: 10.1155/2015/601767

Figure Lengend Snippet: Zif268-positive cells in the IC. (a) Zif268-positive cells in the IC of a sham-operated rat. (b) Zif268-positive cells in the IC of a nerve-injured rat. (c) Comparison of zif268-positive cells in the nerve-injured and sham groups. Zif268-positive cells increased significantly in the IC after nerve injury ( ∗ P < 0.05). Cell counts are expressed per section.

Article Snippet: After washing the section slides 5 times, the sections were incubated with PBS containing 1% normal horse serum (Vector Laboratories) for 30 minutes and then incubated overnight at 4°C in 0.3% Triton X-100, 2% normal horse serum (Vector Laboratories), and rabbit monoclonal anti-zif268 antibody (1 : 4,000, Santa Cruz Biotechnology).

Techniques:

Journal: Biology of Sex Differences

Article Title: Sex differences in contextual pattern separation, neurogenesis, and functional connectivity within the limbic system

doi: 10.1186/s13293-022-00450-2

Figure Lengend Snippet: Experimental design. A Schematic illustration of experimental timeline: subjects received one intraperitoneal injection of 5-chloro-2'-deoxyuridine on Experimental Day 1 and one intraperitoneal injection of 5-iodo-2'-deoxyuridine on Experimental Day 8. Then, subjects were tested in the contextual pattern separation task for a total of 12 days (Experimental Day 16–28), followed by an activation trial in which the rats were placed in the context previously paired with shock but received no shock (Experimental Day 29). During the contextual pattern separation task, subjects were exposed to two different contexts each day; context A a shock-paired context (context paired with foot shocks) and context A’ a neutral context (context with no foot shock). B Brain regions that were examined for functional connectivity using zif268. ACC cingulate cortex (Cg1), PrL prelimbic cortex, IL infralimbic cortex, lDS lateral dorsal striatum, mDS medial dorsal striatum, NAc nucleus accumbens core, NAs nucleus accumbens shell, LA lateral amygdala, BLA basolateral amygdala, CeA central amygdala, dDG dorsal dentate gyrus, vDG ventral dentate gyrus, dCA1 dorsal cornu ammonis 1, vCA1 ventral cornu ammonis 1, dCA3 dorsal cornu ammonis 3, vCA3 ventral cornu ammonis 3

Article Snippet: Sections were then incubated in a primary antibody solution containing 1:500 rabbit anti-zif268 (Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 goat anti-doublecortin (Santa Cruz Biotechnology, Dallas, TX, USA) 0.3% Triton-X (Sigma-Aldrich) and 3% normal donkey serum (NDS; MilliporeSigma, Burlington, MA, USA) in 0.1 M TBS for 24 h at 4 °C.

Techniques: Injection, Activation Assay, Functional Assay

Journal: Biology of Sex Differences

Article Title: Sex differences in contextual pattern separation, neurogenesis, and functional connectivity within the limbic system

doi: 10.1186/s13293-022-00450-2

Figure Lengend Snippet: Sex differences in zif268 activation of dentate adult-born cells. Photomicrographs of doublecortin-immunoreactive (ir) cells (DCX-ir: green) and zif268-ir cells (red) were taken under Zeiss Axio Scan.Z1 with a 40 × objective lens ( E ). Photomicrographs of IdU-ir cells (green) and zif268-ir cells (white) were taken under Olympus FV1000 confocal microscope with a 40 × objective lens ( F ). Photomicrographs of CldU-ir cells (red) and zif268-ir (white) cells were taken under Olympus FV1000 confocal microscope with a 40 × objective lens ( G ). Scale bars indicate 50 μm. Mean (± SEM) percentage of double-labeled cells in the dorsal ( H ) and ventral ( I ) dentate gyrus. Females, compared to males, had greater percentage of IdU/zif268-ir cells in the dorsal and ventral dentate gyrus, whereas there was a significant interaction effect of sex by region for the percentage of CldU/zif268-ir cells, with females having a greater percentage of CldU/zif268 in the dorsal dentate gyrus but males having a greater percentage of CldU/zif268-ir in the ventral dentate gyrus. Mean (± SEM) density of double-labeled cells in the dorsal ( J ) and ventral ( K ) dentate gyrus. There was a significant interaction effect of sex by region for the density of CldU/zif268-ir cells, with females having a greater density of CldU/zif268-ir in the dorsal dentate gyrus but males having a greater density in of CldU/zif268-ir in the ventral dentate gyrus. *Indicates p < 0.05

Article Snippet: Sections were then incubated in a primary antibody solution containing 1:500 rabbit anti-zif268 (Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 goat anti-doublecortin (Santa Cruz Biotechnology, Dallas, TX, USA) 0.3% Triton-X (Sigma-Aldrich) and 3% normal donkey serum (NDS; MilliporeSigma, Burlington, MA, USA) in 0.1 M TBS for 24 h at 4 °C.

Techniques: Activation Assay, Microscopy, Labeling

Journal: Biology of Sex Differences

Article Title: Sex differences in contextual pattern separation, neurogenesis, and functional connectivity within the limbic system

doi: 10.1186/s13293-022-00450-2

Figure Lengend Snippet: Sex differences in zif268 activation in the brain, and correlations with fear response. Photomicrographs of zif268 immunoreactivity in the frontal cortex ( A ) and in the dorsal hippocampus ( B ) in male (left) and female rats (right). Mean (± SEM) density of zif268-ir cells in the frontal cortex ( C ) and in the dorsal hippocampus ( D ). Females, compared to males, had greater density of zif268-ir cells in the anterior cingulate cortex (ACC), in the prelimbic cortex (PrL) and in the dorsal CA1. *Indicates p < 0.05. Heat maps generated based on correlations coefficients between the amount of freezing and activated cells (zif268-ir) in different regions of the limbic system ( E ). Males and females had significant correlations of zif268-ir cell density in different brain regions with the percentage of freezing (*indicates correlations with p < 0.05). There were significant sex differences in the correlations between the percentage of freezing and the density of zif268-ir cells in four different brain regions (Green boxes indicates significant sex differences with p < 0.05). Scatter plots for correlations between the percentage of freezing and the density of zif268-ir cells in the dorsal CA1 (dCA1) ( F ) and in the lateral dorsal striatum (lDS) ( G ), and correlations between the discrimination index on the last trial day and the percentage of IdU/zif268-ir cells in the dorsal dentate gyrus ( H ) in males (blue) and females (red)

Article Snippet: Sections were then incubated in a primary antibody solution containing 1:500 rabbit anti-zif268 (Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 goat anti-doublecortin (Santa Cruz Biotechnology, Dallas, TX, USA) 0.3% Triton-X (Sigma-Aldrich) and 3% normal donkey serum (NDS; MilliporeSigma, Burlington, MA, USA) in 0.1 M TBS for 24 h at 4 °C.

Techniques: Activation Assay, Generated

Journal: Biology of Sex Differences

Article Title: Sex differences in contextual pattern separation, neurogenesis, and functional connectivity within the limbic system

doi: 10.1186/s13293-022-00450-2

Figure Lengend Snippet: Sex differences in inter-regional correlations of zif268-ir cell density. A A heatmap showing correlation coefficients ( r ) of the density of zif268-ir cells between each brain region in males and females. Males and females showed distinct patterns of significant inter-regional correlations of zif268-ir cell density. * Indicates significant correlations ( p < 0.05) and green boxes indicate sex differences between the correlations ( p < 0.05). B Brain network maps were generated with correlations with coefficients larger than 0.67 or smaller than − 0.67 in males (left) and females (right) with p < 0.1. Red lines indicate positive correlations with wider lines indicating larger coefficients and blue lines indicate negative correlations with wider lines indicating smaller coefficients

Article Snippet: Sections were then incubated in a primary antibody solution containing 1:500 rabbit anti-zif268 (Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 goat anti-doublecortin (Santa Cruz Biotechnology, Dallas, TX, USA) 0.3% Triton-X (Sigma-Aldrich) and 3% normal donkey serum (NDS; MilliporeSigma, Burlington, MA, USA) in 0.1 M TBS for 24 h at 4 °C.

Techniques: Generated

Journal: Biology of Sex Differences

Article Title: Sex differences in contextual pattern separation, neurogenesis, and functional connectivity within the limbic system

doi: 10.1186/s13293-022-00450-2

Figure Lengend Snippet: A – C Sex difference in the principal component analysis (PCA). Factor coordinates of the variables in the first three factors identified using principal component analysis (PCA) for zif268 activation ( A ). Scree plot for eigenvalues with the total percentage of variance for the first three factors ( B ). Eigenvalues for the first three factors were significant based on Horn’s parallel analysis. The first three factors explained 70.45% of the variances. A graph showing sex difference in the factor scores of individual samples ( C ). ANOVA and post hoc revealed males and females showed significant sex difference in the first factor. *Indicates p < 0.05 and ** indicates p < 0.01

Article Snippet: Sections were then incubated in a primary antibody solution containing 1:500 rabbit anti-zif268 (Santa Cruz Biotechnology, Dallas, TX, USA), 1:500 goat anti-doublecortin (Santa Cruz Biotechnology, Dallas, TX, USA) 0.3% Triton-X (Sigma-Aldrich) and 3% normal donkey serum (NDS; MilliporeSigma, Burlington, MA, USA) in 0.1 M TBS for 24 h at 4 °C.

Techniques: Activation Assay